Johnny Test (2005)

The animated series "Johnny Test" does not feature any identifiable LGBTQ+ characters or themes within its narrative. The show primarily focuses on the adventures of Johnny, his super-genius sisters, and their various inventions and escapades.

Female characters in Johnny Test, such as Susan and Mary Test, primarily rely on their scientific inventions and intellectual abilities. There are no instances where a female character defeats one or more male opponents through skill, strength, or martial arts in direct physical combat.

Johnny Test is an original animated series, not an adaptation of pre-existing material. All characters were created for the show, establishing their genders from inception. Therefore, no characters were canonically established as a different gender in prior source material.

Johnny Test (2005–2014) is an original animated series, not an adaptation or reboot of a prior work with pre-established character races. All characters were created for this specific show, meaning there was no prior canonical depiction from which their race could have been swapped.