Evangelion: 1.0 You Are (Not) Alone (2007)

Evangelion: 1.0 You Are (Not) Alone does not feature any identifiable LGBTQ+ characters or themes. The film primarily focuses on Shinji Ikari's introduction to NERV, his initial struggles piloting an Evangelion, and battles against the Angels, without exploring queer identities or relationships within its narrative.

The film primarily features combat between giant mechs (Evangelions) piloted by humans and monstrous entities (Angels). Female characters like Rei Ayanami pilot these mechs, but do not engage in or win direct physical combat against male human opponents.

The film is a retelling of the original anime series, and all established characters who appear retain their canonical genders from the source material. No characters were portrayed with a different gender.

This anime film is a remake of an existing Japanese series. All major characters retain their established racial depictions from the original source material, with no instances of a character's race being changed.