Alvin and the Chipmunks: The Squeakquel (2009)

The film "Alvin and the Chipmunks: The Squeakquel" does not feature any discernible LGBTQ+ characters or themes. Its plot centers on the musical adventures and school experiences of the titular chipmunks, leading to a net impact of N/A regarding LGBTQ+ portrayal.

The film does not feature any scenes where a female character engages in and wins direct physical combat against one or more male opponents. The Chipettes' roles are primarily musical and comedic, without physical confrontations of this nature.

All established characters, including Alvin, Simon, Theodore, and Dave, maintain their canonical genders. The Chipettes, while new to this film series, are established female characters from the broader franchise and are not gender-swapped versions of any prior male characters.

The film features anthropomorphic chipmunks and human characters. The chipmunks' species does not align with human racial categories. All human characters are portrayed by actors of the same race as their established or original portrayals, with no instances of a character canonically established as one race being portrayed as another.